T7

Part:BBa_K5250002:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-09-28)


T7-like RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 109
    Illegal BsaI site found at 1173
    Illegal BsaI site found at 2043
    Illegal BsaI.rc site found at 61
    Illegal BsaI.rc site found at 553
    Illegal BsaI.rc site found at 1481
    Illegal BsaI.rc site found at 2491
    Illegal SapI site found at 2472
    Illegal SapI.rc site found at 1667


Design Notes

The T7-like RNAP gene is large and can be challenging to clone using the Golden Gate assembly.

Source

Sequence is derived from the Pseudomonas phage Pf-10: https://ncbi.nlm.nih.gov/datasets/genome/GCF_001038595.1/

References

Lammens, E., Feyaerts, N., Kerremans, A., Boon, M., & Lavigne, R. (2023). Assessing the orthogonality of Phage-Encoded RNA polymerases for tailored synthetic biology applications in pseudomonas species. International Journal of Molecular Sciences, 24(8), 7175. https://doi.org/10.3390/ijms24087175